The Sepax TweenTrap Column is specifically designed for trapping surfactants, such as Polysorbate 80/20 (PS 80/20, Tween 80/20), in aqueous samples. The packing support is composed of rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) beads with a particle size of 5 μm and a proprietary surface coating is covalently bonded onto the PS/DVB support. TweenTrap columns can be run in tandem with SEC columns in aqueous solutions, and are specifically designed to trap and remove surfactants, such as Polysorbate 80/20, which can interfere with quantitative protein SEC analysis.A case study using our TweenTrap column, along with our SEC column and Erbitux mAb containing 0.04% Tween 80 sample is shown below. Our TweenTrap columns helps to minimize the interference of tween in the reporting of dimer versus monomer species of Erbitux, a commercially available recombinant, monoclonal antibody (mAb).
